From December 14, 2017 to April 12, 2019
This study aimed to cultivate H.ostrearia in different media: seawater (SW), seawater filtered by activated carbon (SW+CA), and drilling water (DW). The three water media were supplemented with two types of nutrients such as Walne-Conway+silica (CS) and ES-1/3 Provasoli (P) leading to six experiment series. The semi-continuous modes condition were used in the short-term cultures in order to gain an optimal growth condition of living cells and production of Extracellular marennine (Emn) and Intracellular marennine (Imn) by measuring their pigment concentration. The cultures were grown in 200 mL and 400 mL. The dilution cycle was every 3-4 days for 200 mL volume and 4-5 days for 400 mL volume during 20-40 days or until the cultures showed a slower growth. Cell growth was observed by using inverted-microscope and followed by calculated cell densities with Nageotte hemocytometer. The concentration of Emn and Imn was determined spectrophotometrically.
