From August 31, 2018 to November 1, 2018
During my secondment at Le Mans University in chemistry and biology laboratory, I made experiments of characterization of mareninne structure by enzyme hydrolysis.
The enzymes used in these experiments were of the glucanase family. I used a microplate reader and a UV-visible spectrophotometer to monitor the enzyme hydrolysis. I also used the big scale enzyme hydrolysis for cellulase by incubation at 37˚C overnight. After that, the supernatant was dialyzed with a membrane and the dialysates outside the membrane were evaporated until dryness and dissolved in D2O to perform the proton NMR. The NMR results showed that there were some peak around 3-4 ppm, in the monosaccharide region.
