Two new Bacillariodnaviruses associated with the marine diatom Haslea ostrearia

Romain Gastineau1*, Claude Lemieux2, Monique Turmel2, Frederic Verret3, Notis Argiriou4, Gregory Carrier5, Jean-Luc Mouget1

 

1 FR CNRS 3473 IUML, Mer-Molécules-Santé (MMS), Université du Maine, Avenue Olivier Messiaen, 72085 Le Mans CEDEX 9, France

2 Département de biochimie, de microbiologie et de bio-informatique, Institut de Biologie Intégrative et des Systèmes, Université Laval, Québec, Québec, Canada

3 Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion, Crete, Greece

4 Institute of Agrobiotechnology, CERTH, Thermi GR-570 01, Greece

5 IFREMER, PBA, Rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France

 

Keywords: Haslea ostrearia, new diatom infecting viruses, NGS, oyster ponds, phylogeny

 

The complete genomes of two new Bacillariodnaviruses associated with the blue diatom Haslea ostrearia have been sequenced. They received the name V-148 and V-235. Their genomes are shorter (4567 and 4538 bp) than those of other diatom infecting viruses, and only two conserved open reading frames have been evidenced. Phylogenetic analysis associates them with Bacillariodnavirus LDMD-2013 and viruses infecting species from the genus Chaetoceros. While V-148 and V-235 have been found associated with three distinct clones of H. ostrearia with a similar region of origin (oyster ponds from the Bay of Bourgneuf, France), an in silico research conducted on the DNA of an Australian sub-population of H. ostrearia and of other species from the same genus has been unsuccessful, suggesting that these viruses may be endemic to the Bay of Bourgneuf.