Prophylactic effect of Haslea ostrearia culture supernatant containing the pigment marennine to stabilize bivalve hatchery production

Réjean Tremblay1, François Turcotte1, Bertrand Genard2, Karine Lemarchand1, Jean-Sebastien Deschênes1, Jean-Luc Mouget3

 

1 Institut des Sciences de la mer de Rimouski, Université du Québec à Rimouski, 310 des Ursulines, Rimouski, QC G5L 3A1, Canada.

2 Iso-Biokem, 112, 5e Tang Ouest, ST-Joseph-De-Lepage, G5H 3K6, Canada

3 FR CNRS 3473 IUML, Mer-Molécules-Santé (MMS), Université du Maine, Avenue O. Messiaen, 72085 Le Mans Cedex 9, France

 

Keywords : microalgal by-products, Mytilus edulis, Placopecten magellanicus, Vibrio splendidus


This paper explores the possibility of using the supernatant of Haslea ostrearia culture containing marennine, a natural microalgal pigment, as an antimicrobial in bivalve hatcheries [1]. The use of known antibiotics to control diseases is generally avoided in hatcheries, as they are expensive, may leave harmful compounds, and may cause the development of antibiotic-resistant bacterial strains. Hence, there is a need to identify new molecules with antimicrobial activities [2] and marennine could be such a molecule. The blue mussel Mytilus edulis and the scallop Placopecten magellanicus were used as model animals, and the pathogenic marine bacteria Vibrio splendidus was used to induce larval mortality. The hypothesis tested was that V. splendidus pathogenicity in larval rearings can be controlled by using marennine-containing culture supernatants. The effect of three marennine concentrations was tested on a larval rearing over 20 days for M. edulis and 9 days for P. magellanicus. At a low dose (0.1 mg L−1), survival and physiological condition were both higher than in the control. In bacterial challenges, larvae were exposed to V. splendidus for 72 h, with or without marennine. The bacterial challenge caused significant mortality when compared to controls, while the marennine-treated larvae showed significantly higher survival. Results show that marennine is an interesting molecule for pathogen control in hatcheries as it is active at low concentrations and significantly enhanced larval survival and physiological condition [3].



[1] Gastineau, R., Turcotte, F., Pouvreau, J.-B., Morançais, M., Fleurence, J., Windarto, E., Prasetiya, F., Arsad, S., Jaouen, P., Babin, M., Coiffard, L., Couteau, C., Bardeau, J.-F., Jacquette, B., Leignel, V., Hardivillier, Y., Marcotte, I., Bourgougnon, N., Tremblay, R., Deschênes, J.-S., Badawy, H., Pasetto, P., Davidovich, N., Hansen, G., Dittmer, J., Mouget, J.-L., 2014. Marennine, promising blue pigments from a widespread Haslea diatom species complex. Marine Drugs. 12, 3161-3189.


[2] Falaise, C., François, C., Travers, M.-A., Morga, B., Haure, J., Tremblay, R., Turcotte, F., Pasetto, P., Gastineau, R., Hardivillier, Y., Leignel, V., Mouget, J.-L., 2016. Antimicrobial Compounds from Eukaryotic Microalgae against Human Pathogens and Diseases in Aquaculture. Marine Drugs. 14, 159.


[3] Turcotte, F., Mouget, J.-L., Genard, B., Lemarchand, K., Deschênes, J.-S., Tremblay, R., 2016. Prophylactic effect of Haslea ostrearia culture supernatant containing the pigment marennine to stabilize bivalve hatchery production. Aquat. Living Resour. 29, 401.